2VFI
Crystal structure of the Plasmodium falciparum triosephosphate isomerase in the loop closed state with 3-phosphoglycerate bound at the active site and interface
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-12-10 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.611, 51.135, 92.185 |
Unit cell angles | 90.00, 98.24, 90.00 |
Refinement procedure
Resolution | 91.290 - 2.250 |
R-factor | 0.21167 |
Rwork | 0.209 |
R-free | 0.26022 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1o5x |
RMSD bond length | 0.006 |
RMSD bond angle | 0.968 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.100 | 0.380 |
Number of reflections | 22598 | |
<I/σ(I)> | 9.2 | 2.3 |
Completeness [%] | 95.1 | 80.5 |
Redundancy | 2.6 | 1.82 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 0.1 M SODIUM ACETATE PH 4.0-5.5 PEG 1450 8-24% |