2VFD
Crystal structure of the F96S mutant of Plasmodium falciparum triosephosphate isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-20 |
| Detector | DIP-2040B |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.423, 50.698, 89.188 |
| Unit cell angles | 90.00, 92.17, 90.00 |
Refinement procedure
| Resolution | 36.760 - 1.400 |
| R-factor | 0.16628 |
| Rwork | 0.165 |
| R-free | 0.18828 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1o5x |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.088 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.050 | 0.240 |
| Number of reflections | 90838 | |
| <I/σ(I)> | 40.5 | 7 |
| Completeness [%] | 96.3 | 93.9 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1 M SODIUM ACETATE PH 4.0-5.5, PEG 1450 8-24% |
