2VEC
The crystal structure of the protein YhaK from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.648, 57.648, 162.092 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.750 - 1.850 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.670 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.420 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.080 | 0.550 |
| Number of reflections | 23745 | |
| <I/σ(I)> | 28 | 4.3 |
| Completeness [%] | 97.8 | 92.3 |
| Redundancy | 19.3 | 19.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1 M CITRIC ACID, PH 5.0, 2.0 M NACL |
