2VEA

The complete sensory module of the cyanobacterial phytochrome Cph1 in the Pr-state.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-13
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	77.180, 77.180, 249.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.680 - 2.210
R-factor	0.245
Rwork	0.244
R-free	0.27100
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.007
RMSD bond angle	1.301
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.050	0.360
Number of reflections	26939
<I/σ(I)>	23	3.5
Completeness [%]	67.2	6.7
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.7		TETRAGONAL CRYSTALS OF THE CPH1 PR STATE WERE GROWN IN 96-WELL CRYSTALLIZATION PLATES USING DROPS COMPRISING 500 NL 10 MG/ML CPH1-DELTA2 IN 2.5 MM TRIS/HCL PH 7.8, 15 MM NACL PLUS 500 NL RESERVOIR SOLUTION (2.1 M SODIUM ACETATE, PH 6.7).