2VE4
Substrate free cyanobacterial CYP120A1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.640, 122.170, 64.920 |
| Unit cell angles | 90.00, 115.00, 90.00 |
Refinement procedure
| Resolution | 19.610 - 2.400 |
| R-factor | 0.234 |
| Rwork | 0.230 |
| R-free | 0.30400 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.441 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.080 |
| Number of reflections | 40633 |
| <I/σ(I)> | 11.6 |
| Completeness [%] | 95.5 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 5% ETHYLENE GLYCOL, 7% PEG 3350, 0.1 M TRIS PH7.5 |
