2VE3
Retinoic acid bound cyanobacterial CYP120A1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.460, 132.970, 67.420 |
| Unit cell angles | 90.00, 114.50, 90.00 |
Refinement procedure
| Resolution | 19.850 - 2.100 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SUBSTRATE-FREE CYP120A1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.370 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.100 |
| Number of reflections | 62855 |
| <I/σ(I)> | 9.6 |
| Completeness [%] | 95.0 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.2 M SODIUM POTASSIUM TARTATE, 0.1 M MES PH6.5 |
