2VDT
Crystallographic structure of Levansucrase from Bacillus subtilis mutant S164A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-13 |
| Detector | RIGAKU-MSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.880, 55.800, 124.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.410 - 3.200 |
| R-factor | 0.2645 |
| Rwork | 0.265 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oyg |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.410 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.140 | 0.440 |
| Number of reflections | 5627 | |
| <I/σ(I)> | 9 | 1.8 |
| Completeness [%] | 91.7 | 94.9 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7 | CRYSTALS WERE OBTAINED AFTER 12 DAYS OF MICRODIALYSIS OF THE PURIFIED PROTEIN (8 G/L) AGAINST DEIONIZED WATER., pH 7 |
