2VDJ
Crystal Structure of Homoserine O-acetyltransferase (metA) from Bacillus Cereus with Homoserine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 96.390, 96.390, 75.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.470 - 2.000 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ghr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.987 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.510 |
| Number of reflections | 22903 | |
| <I/σ(I)> | 16.85 | 2.59 |
| Completeness [%] | 93.0 | 99.5 |
| Redundancy | 6 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 1.6M AMMONIUM SULFATE, 0.1 M TRIS, PH 8, 10MM HOMOSERINE |
