2VDC
THE 9.5 A RESOLUTION STRUCTURE OF GLUTAMATE SYNTHASE FROM CRYO-ELECTRON MICROSCOPY AND ITS OLIGOMERIZATION BEHAVIOR IN SOLUTION: FUNCTIONAL IMPLICATIONS.
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | JEOL 2010UHR |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 0.5 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 151 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 9.5 |
| Software | UCSF Chimera,SPIDER |
| Symmetry | I |
