2VD2

The crystal structure of HisG from B. subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF
Synchrotron site	ESRF
Temperature [K]	100
Spacegroup name	P 43 2 2
Unit cell lengths	85.937, 85.937, 75.948
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.570 - 2.850
R-factor	0.237
Rwork	0.236
R-free	0.26600
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.013
RMSD bond angle	1.642
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Rmerge	0.080
Number of reflections	36000
<I/σ(I)>	12
Completeness [%]	89.0
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1