2VCP
Crystal structure of N-Wasp VC domain in complex with skeletal actin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 136.240, 136.240, 205.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.275 |
| Rwork | 0.275 |
| R-free | 0.33100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a3z |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.120 | 0.400 |
| Number of reflections | 35056 | |
| <I/σ(I)> | 8.8 | 2.3 |
| Completeness [%] | 98.5 | 95 |
| Redundancy | 3.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | VAPOR DIFFUSION METHOD (4 C) PROTEIN SOLUTION: 0.18MM ACTIN, 0.36MM N-WASP PEPTIDE, 5MM TRIS.HCL PH7.0, ATP 0.2MM, CACL2 0.02MM, TCEP 20MM, NAN3 0.01%. RESERVOIR: 10.2% (V/V) TACSIMATE, 13.2%(W/V)PEG 8000, 100MM HEPES PH7.0 |
