2VCK
Structure of Phycoerythrobilin Synthase PebS from the Cyanophage P-SSM2 in Complex with the bound Substrate Biliverdin IXa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.560, 67.570, 81.280 |
| Unit cell angles | 92.26, 109.07, 106.58 |
Refinement procedure
| Resolution | 49.210 - 1.800 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vcl |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.468 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.110 | 0.420 |
| Number of reflections | 91993 | |
| <I/σ(I)> | 13.2 | 5 |
| Completeness [%] | 97.1 | 95.9 |
| Redundancy | 8.1 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | HANGING DROP VAPOUR DIFFUSION PROTEIN:RESERVOIR 2:1, pH 5.5 |
