2VBX
Feast or famine regulatory protein (Rv3291c)from M. tuberculosis complexed with L-Phenylalanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Detector | MARRESEARCH |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 100.899, 100.899, 99.286 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.430 - 2.750 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.27000 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.110 | 0.570 |
| Number of reflections | 13598 | |
| <I/σ(I)> | 13.7 | 2.9 |
| Completeness [%] | 98.6 | 99.7 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
