2VBW

Feast or famine regulatory protein (Rv3291c)from M. tuberculosis complexed with L-Phenylalanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	MARRESEARCH
Spacegroup name	P 42 21 2
Unit cell lengths	100.954, 100.954, 99.307
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.430 - 2.200
R-factor	0.213
Rwork	0.211
R-free	0.25400
Structure solution method	MIRAS
Starting model (for MR)	NONE
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.100	0.760
Number of reflections	26683
<I/σ(I)>	11.9	2.2
Completeness [%]	99.9	100
Redundancy	5.1	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1