2VBN
Molecular basis of human XPC gene recognition and cleavage by engineered homing endonuclease heterodimers
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.115, 67.992, 77.186 |
Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
Resolution | 46.130 - 1.900 |
R-factor | 0.145 |
Rwork | 0.141 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g9z |
RMSD bond length | 0.013 |
RMSD bond angle | 2.059 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.130 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.070 |
Number of reflections | 33531 |
Completeness [%] | 93.5 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 4 MG/ML PROTEIN, 20% PEG1000, 0.1M IMIDAZOLE PH 8.0, 0.2 M CAAC2 |