2VBC
Crystal structure of the NS3 protease-helicase from Dengue virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.866, 88.242, 76.676 |
| Unit cell angles | 90.00, 94.21, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.150 |
| R-factor | 0.206 |
| Rwork | 0.202 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2FOM AND 2BMF |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.088 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.300 | 3.300 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.140 | 0.680 |
| Number of reflections | 12236 | |
| <I/σ(I)> | 11 | 2.1 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
