2VB1
HEWL at 0.65 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.070, 31.250, 33.760 |
| Unit cell angles | 87.98, 108.00, 112.11 |
Refinement procedure
| Resolution | 30.000 - 0.650 |
| R-factor | 0.0848 |
| R-free | 0.09520 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.024 |
| RMSD bond angle | 0.060 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | ACORN |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 0.670 |
| High resolution limit [Å] | 0.650 | 0.650 |
| Rmerge | 0.040 | 0.180 |
| Number of reflections | 187165 | |
| <I/σ(I)> | 36.2 | 4.2 |
| Completeness [%] | 97.6 | 67.3 |
| Redundancy | 7.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | SITTING DROP METHOD. PROTEIN SOLUTION: 35 MG/ML HEWL, 0.02 M NA ACETATE BUFFER PH 4.7 WELL SOLUTION: 1 M NANO3, 0.1 M NA ACETATE BUFFER PH 4.7, 20 % ETHYLENE GLYCOL PROTEIN AND WELL SOLUTIONS MIXED 1:1 IN A DROP AND SEEDED |
