2VAR
Crystal structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase complexed with 2-keto-3-deoxygluconate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 104.553, 104.553, 420.782 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 61.660 - 2.100 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.700 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.500 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.080 | 0.310 |
Number of reflections | 80339 | |
<I/σ(I)> | 15.7 | 4 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 6.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |