2VAA

MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN

Replaces:  1VAA

Experimental procedure

Detector technology	AREA DETECTOR
Detector	XENTRONICS
Spacegroup name	P 21 21 2
Unit cell lengths	138.100, 88.900, 45.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.171
Rwork	0.171
RMSD bond length	0.007
RMSD bond angle	1.900
Data reduction software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.079
Total number of observations	81010 *
Number of reflections	23289
Completeness [%]	90.0
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.25 *	22.5 *	used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	H-2Kb-peptide complex	4-6 (mg/ml)
2	1	drop	sodium potassium phosphate	1.84 (M)
3	1	drop	MPD	2 (%)