2VA8

DNA Repair Helicase Hel308

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-12
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	61.657, 138.083, 107.551
Unit cell angles	90.00, 94.65, 90.00

Refinement procedure

Resolution	107.210 - 2.300
R-factor	0.214
Rwork	0.212
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TRACED FROM MAD DATASET
RMSD bond length	0.011
RMSD bond angle	1.182
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.078	0.309
Number of reflections	79222
<I/σ(I)>	14.7	4.2
Completeness [%]	99.0	99.3
Redundancy	6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5		15.3% PEG8000, 0.1M SODIUM CACODYLATE, PH6.5, 0.13M AMMONIUM SULFATE AND 0.03M MAGNESIUM CHLORIDE
1	VAPOR DIFFUSION, HANGING DROP	6.5		15.3% PEG8000, 0.1M SODIUM CACODYLATE, PH6.5, 0.13M AMMONIUM SULFATE AND 0.03M MAGNESIUM CHLORIDE