2V9V
Crystal Structure of Moorella thermoacetica SelB(377-511)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-21 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.349, 46.087, 58.683 |
| Unit cell angles | 90.00, 120.45, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.100 |
| R-factor | 0.121 |
| Rwork | 0.120 |
| R-free | 0.14100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RESIDUES 377-511 OF PDB ENTRY 1LVA |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.548 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.200 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.090 | 0.250 |
| Number of reflections | 61009 | |
| <I/σ(I)> | 10 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 100 MM HEPES-NAOH, PH 6.9 TO 7.9, 3.9 TO 4.5 M NACL, HANGING DROP VAPOR DIFFUSION AT 293K |
