2V9S

Second LRR domain of human Slit2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-08
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	60.360, 123.450, 127.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.216
Rwork	0.213
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.662
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.120	0.360
Number of reflections	61528
<I/σ(I)>	10.2	4.6
Completeness [%]	94.3	87.1
Redundancy	5.9	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		20-24% PEG 4000, 20% ISOPROPANOL, 0.1M NA CITRATE, PH=5.6