2V9M

L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT A87M- T109F-E192A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-14
Detector	MARRESEARCH
Spacegroup name	P 4
Unit cell lengths	83.407, 83.407, 97.299
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.300
R-factor	0.1044
R-free	0.14050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ojr
RMSD bond length	0.013
RMSD bond angle	0.031
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	EPMR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.300	1.300
Rmerge	0.060	0.400
Number of reflections	161435
<I/σ(I)>	20.88	5.39
Completeness [%]	99.9	100
Redundancy	8.38	8.26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4		40% (V/V) ETHYLENE GLYCOL, 0.1 M TRIS (PH 7.0)