2V9L

L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT Q6Y- E192A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-14
Detector	MARRESEARCH
Spacegroup name	P 4 21 2
Unit cell lengths	107.059, 107.059, 57.161
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.230
R-factor	0.0904
R-free	0.12210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ojr
RMSD bond length	0.015
RMSD bond angle	0.035
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	EPMR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.230	1.230
Rmerge	0.060	0.360
Number of reflections	96326
<I/σ(I)>	20	6.15
Completeness [%]	99.4	100
Redundancy	8.81	8.83

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5		40% (V/V) 1,2-PROPANEDIOL, 0.1 M ACETATE (PH4.5), pH 5.0