2V9E
L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT E192A- K248W-A273S)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 107.520, 107.520, 115.017 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.880 - 1.580 |
| R-factor | 0.159 |
| Rwork | 0.158 |
| R-free | 0.17600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ojr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.396 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 91205 | |
| <I/σ(I)> | 19.33 | 5.96 |
| Completeness [%] | 98.6 | 99.8 |
| Redundancy | 8.2 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 40% (V/V) PEG 400, 0.1M HEPES (PH 7.5), 0.2 M CALCIUM ACETATE |
