2V9A
Structure of Citrate-free Periplasmic Domain of Sensor Histidine Kinase CitA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-08 |
Detector | MARRESEARCH |
Spacegroup name | I 4 |
Unit cell lengths | 64.163, 64.163, 144.746 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.663 - 2.000 |
Rwork | 0.212 |
R-free | 0.27830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j80 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.050 | 0.190 |
Number of reflections | 19151 | |
<I/σ(I)> | 16.7 | |
Completeness [%] | 97.0 | 84.1 |
Redundancy | 5.49 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HANGING DROP, VAPOUR DIFFUSION, RESERVOIR: 20MM HEPES,PH 7.5,0.63M NAH2PO4,0.63M KH2PO4, PROTEIN SOLUTION: 15 MG/ML,MIXING RATIO 1:1,TEMPERATURE 293K |