2V8Z
Crystal Structure of YagE, a prophage protein belonging to the dihydrodipicolinic acid synthase family from E. coli K12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-08 |
| Detector | RIGAKU RAXIS-IV |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 141.689, 156.494, 55.884 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.200 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v9d |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.788 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.140 | 0.590 |
| Number of reflections | 73987 | |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 99.4 | 96.3 |
| Redundancy | 6.7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100 MM HEPES PH 6.5, 200 MM MGCL2, 25% PEG 3350, RT |
