2V8X
Crystallographic and mass spectrometric characterisation of eIF4E with N7-cap derivatives
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.256, 100.213, 135.209 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.100 - 2.300 |
R-factor | 0.192 |
Rwork | 0.188 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ejh |
RMSD bond length | 0.018 |
RMSD bond angle | 1.753 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.100 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.120 | 0.470 |
Number of reflections | 21598 | |
<I/σ(I)> | 5.8 | 1.6 |
Completeness [%] | 91.0 | 87 |
Redundancy | 3.6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 25% PEG 6000, 7% AMMONIUM SULPHATE, 100MM HEPES-KOH PH 7.0 |