2V8T
Crystal structure of Mn catalase from Thermus Thermophilus complexed with chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 132.070, 132.070, 132.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 0.980 |
| R-factor | 0.101 |
| Rwork | 0.101 |
| R-free | 0.11700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v8u |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 0.990 |
| High resolution limit [Å] | 0.980 | 0.980 |
| Rmerge | 0.040 | 0.250 |
| Number of reflections | 413309 | |
| <I/σ(I)> | 30 | 4 |
| Completeness [%] | 98.5 | 98 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
