2V8S

VTI1B HABC DOMAIN - EPSINR ENTH DOMAIN COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-01
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	102.231, 60.935, 40.998
Unit cell angles	90.00, 94.09, 90.00

Refinement procedure

Resolution	19.550 - 2.220
R-factor	0.201
Rwork	0.196
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	UNCOMPLEXED STRUCTURES
RMSD bond length	0.025
RMSD bond angle	2.046
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	2.330
High resolution limit [Å]	2.200	2.200
Rmerge	0.070	0.360
Number of reflections	11925
<I/σ(I)>	18.7	4.5
Completeness [%]	97.8	89.9
Redundancy	5.2	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1