2V8O
Structure of the Murray Valley encephalitis virus RNA helicase to 1. 9A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-16 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.516, 76.414, 70.609 |
Unit cell angles | 90.00, 91.73, 90.00 |
Refinement procedure
Resolution | 70.530 - 1.900 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bmf |
RMSD bond length | 0.009 |
RMSD bond angle | 1.219 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CaspR |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.080 | 0.550 |
Number of reflections | 36088 | |
<I/σ(I)> | 27.6 | 2.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.1 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 5% PEG6000, 0.1M HEPES PH 7.0 |