2V8O
Structure of the Murray Valley encephalitis virus RNA helicase to 1. 9A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.516, 76.414, 70.609 |
| Unit cell angles | 90.00, 91.73, 90.00 |
Refinement procedure
| Resolution | 70.530 - 1.900 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bmf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.219 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CaspR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.550 |
| Number of reflections | 36088 | |
| <I/σ(I)> | 27.6 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 5% PEG6000, 0.1M HEPES PH 7.0 |
