2V8L
Carbohydrate-binding of the starch binding domain of Rhizopus oryzae glucoamylase in complex with beta-cyclodextrin and maltoheptaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 2006-04-19 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.591, 42.725, 70.064 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.360 - 1.800 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2djm |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.030 | 0.440 |
Number of reflections | 12310 | |
<I/σ(I)> | 41.3 | 2.25 |
Completeness [%] | 99.1 | 94.1 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | PEG8K, ZINC ACETATE, pH 6.5 |