2V8D
Crystal structure of mutant E159A of beta-alanine synthase from Saccharomyces kluyveri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.117, 77.143, 108.228 |
| Unit cell angles | 90.00, 97.12, 90.00 |
Refinement procedure
| Resolution | 45.220 - 2.300 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r43 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.131 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.460 |
| Number of reflections | 43156 | |
| <I/σ(I)> | 11.9 | 2 |
| Completeness [%] | 97.0 | 88 |
| Redundancy | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | TRISODIUM CITRATE, DIOXANE, ACETONE, DTT, PH 6.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
