2V87
Crystal structure of RAG2-PHD finger in complex with H3R2me2sK4me3 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-08-17 |
Detector | RIGAKU-MSC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.407, 46.630, 57.005 |
Unit cell angles | 90.00, 95.13, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.181 |
Rwork | 0.181 |
R-free | 0.20810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v83 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.811 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.060 | 0.210 |
Number of reflections | 19094 | |
<I/σ(I)> | 25.5 | 3.9 |
Completeness [%] | 96.5 | 87.9 |
Redundancy | 2.6 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | VAPOR DIFFUSION. HANGING DROP. 22% PEG 3350, 120-240 MM OF POTASSIUM FORMATE. TEMPERATURE 293K |