2V7Z
Crystal structure of the 70-kDa heat shock cognate protein from Rattus norvegicus in post-ATP hydrolysis state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.851, 94.792, 78.234 |
| Unit cell angles | 90.00, 93.24, 90.00 |
Refinement procedure
| Resolution | 29.010 - 3.500 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hsc |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.600 |
| Data reduction software | Blu-Ice (CONTROL SOFTWARE) |
| Data scaling software | SCALEPACK (HKL-2000) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.010 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.140 | 0.260 |
| Number of reflections | 15130 | |
| <I/σ(I)> | 10.4 | 5 |
| Completeness [%] | 98.4 | 97.1 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 100 MM CHES, PH 9.5, 23% (W/V) PEG2000, 2% (W/V) PEG8000, 10 MM THREONINE |
