2V70
Third LRR domain of human Slit2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-22 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.650, 96.860, 87.150 |
| Unit cell angles | 90.00, 95.32, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.010 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODIFIED PDB ENTRY 1W8A |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.683 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.500 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.090 | 0.300 |
| Number of reflections | 45165 | |
| <I/σ(I)> | 11.1 | 3.2 |
| Completeness [%] | 91.9 | 94.5 |
| Redundancy | 2.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 15% PEG 4000, 0.2M NH4 ACETATE, 0.1M NACITRATE, PH=5.6. |
