2V6Y
Structure of the MIT domain from a S. solfataricus Vps4-like ATPase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 56.178, 69.267, 123.184 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.660 - 2.400 |
| R-factor | 0.253 |
| Rwork | 0.252 |
| R-free | 0.27700 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.333 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.500 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.370 |
| Number of reflections | 27901 | |
| <I/σ(I)> | 10.3 | 1.9 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | RESERVOIR: 0.6 M AMMONIUM TARTRATE AND 2% PEG4K PROTEIN SOLUTION: 9 MG/ML IN 20 MM TRIS PH 8, 100 MM NACL, 2MM DTT |
