2V6X

Stractural insight into the interaction between ESCRT-III and Vps4

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Detector technology	CCD
Collection date	2007-01-26
Detector	MARRESEARCH
Wavelength(s)	0.8726, 0.9392
Spacegroup name	P 21 21 2
Unit cell lengths	78.145, 82.270, 21.474
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.610 - 1.980
R-factor	0.2
Rwork	0.198
R-free	0.22800
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.015
RMSD bond angle	1.334
Data reduction software	SCALA
Data scaling software	TRUNCATE
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.610	2.020
High resolution limit [Å]	2.000	2.000
Rmerge	0.120	0.440
Number of reflections	9997
<I/σ(I)>	13.4	4.28
Completeness [%]	99.8	100
Redundancy	5.23	5.31

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		0.1M HEPES(PH7.0), 2.0M AMMONIUM SULFATE
1		7		0.1M HEPES(PH7.0), 2.0M AMMONIUM SULFATE