2V6W
tRNASer acceptor stem: Conformation and hydration of a microhelix in a crystal structure at 1.8 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 36.100, 38.920, 30.790 |
| Unit cell angles | 90.00, 110.61, 90.00 |
Refinement procedure
| Resolution | 80.000 - 1.800 |
| Rwork | 0.154 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 80.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.070 |
| Number of reflections | 3677 |
| <I/σ(I)> | 14.9 |
| Completeness [%] | 98.9 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 294 | 50 MM SODIUM CACODYLATE, PH 6.0, 15 MM MAGNESIUM SULFATE, 1.8 M LITHIUM SULFATE, 294 K. |
