2V6M
Crystal structure of lactate dehydrogenase from Thermus Thermophilus HB8 (Apo form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.092, 135.296, 85.645 |
| Unit cell angles | 90.00, 94.04, 90.00 |
Refinement procedure
| Resolution | 29.220 - 2.200 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ldn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 61724 | |
| <I/σ(I)> | 12.29 | 3.38 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 3.78 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1M MES PH 6 15% PEG 6000 |
