2V6C

Crystal structure of ErbB3 binding protein 1 (Ebp1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	298
Detector technology	CCD
Collection date	2005-01-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	49.147, 72.779, 115.624
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.450 - 2.500
R-factor	0.182
Rwork	0.182
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1KQ9 AND 1XGS
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER (1.2)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.400	2.660
High resolution limit [Å]	2.500	2.500
Rmerge	0.080	0.410
Number of reflections	14714
<I/σ(I)>	10.4	3.3
Completeness [%]	98.9	98.9
Redundancy	3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.7		SEE PAPER, pH 7.7