2V6B
Crystal structure of lactate dehydrogenase from Deinococcus Radiodurans (apo form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 123.350, 127.930, 72.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 2.500 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ldn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.550 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 40233 | |
| <I/σ(I)> | 17.06 | 3.12 |
| Completeness [%] | 98.9 | 94.6 |
| Redundancy | 4.35 | 4.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 0.1M BICINE PH 9, 20% PEG 5K MME |
