2V67
Crystal structure of Chlamydomonas reinhardtii Rubisco with a large- subunit supressor mutation T342I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-05-15 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 120.094, 178.558, 122.495 |
Unit cell angles | 90.00, 117.83, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gk8 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.140 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.130 | 0.690 |
Number of reflections | 272649 | |
<I/σ(I)> | 7.5 | 1.3 |
Completeness [%] | 89.2 | 89.9 |
Redundancy | 17.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | pH 7.5 |