2V63
Crystal structure of Rubisco from Chlamydomonas reinhardtii with a large-subunit V331A mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2003-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 121.377, 177.446, 122.568 |
| Unit cell angles | 90.00, 117.65, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gk8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.108 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.230 | 0.460 |
| Number of reflections | 389935 | |
| <I/σ(I)> | 16.5 | 2.4 |
| Completeness [%] | 92.4 | 60 |
| Redundancy | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
