2V5V
W57E Flavodoxin from Anabaena
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.848, 55.576, 70.253 |
| Unit cell angles | 90.00, 91.17, 90.00 |
Refinement procedure
| Resolution | 25.480 - 1.880 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1flv |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.597 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.990 | 1.980 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.050 | 0.190 |
| Number of reflections | 23136 | |
| <I/σ(I)> | 9.5 | 3.6 |
| Completeness [%] | 96.8 | 96.8 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 28 % PEG 4000, 0.1M TRIS/HCL PH 8.5, 0.2M MGCL2 |
