2V5O
STRUCTURE OF HUMAN IGF2R DOMAINS 11-14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.554, 69.247, 98.012 |
| Unit cell angles | 90.00, 103.25, 90.00 |
Refinement procedure
| Resolution | 28.460 - 2.910 |
| R-factor | 0.259 |
| Rwork | 0.257 |
| R-free | 0.30400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v5n |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.164 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 19919 | |
| <I/σ(I)> | 13.9 | 3 |
| Completeness [%] | 98.6 | 92.8 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.4 | 0.1 M MES PH6.4, 2.5% (W/V) PEG 3000 |
