2V5A
CRYSTAL STRUCTURE OF BIOTIN CARBOXYLASE FROM E.COLI IN COMPLEX WITH POTENT INHIBITOR 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Collection date | 2005-10-31 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.315, 106.410, 122.184 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.320 - 2.310 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dv2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.998 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.390 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.070 | 0.380 |
| Number of reflections | 41691 | |
| <I/σ(I)> | 15.99 | 2.74 |
| Completeness [%] | 85.3 | 83 |
| Redundancy | 3.21 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M POTASSIUM CHLORIDE, 4% (W/V) PEG 8000 |
