2V52
Structure of MAL-RPEL2 complexed to G-actin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-11 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.750, 55.440, 138.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.352 - 1.450 |
R-factor | 0.149 |
Rwork | 0.147 |
R-free | 0.18780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.060 |
RMSD bond angle | 2.840 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.530 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.070 | 0.550 |
Number of reflections | 74958 | |
<I/σ(I)> | 6 | 1.2 |
Completeness [%] | 99.3 | 96 |
Redundancy | 5.2 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |