2V4U
Human CTP synthetase 2 - glutaminase domain in complex with 5-OXO-L- NORLEUCINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-21 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.880, 72.330, 41.470 |
| Unit cell angles | 90.00, 95.60, 90.00 |
Refinement procedure
| Resolution | 19.720 - 2.300 |
| R-factor | 0.211 |
| Rwork | 0.207 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vkt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.266 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.250 |
| Number of reflections | 15010 | |
| <I/σ(I)> | 18.27 | 11.3 |
| Completeness [%] | 96.0 | 86.6 |
| Redundancy | 5.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1M HEPES, PH 7.0, 0.02 M MGCL2, 30% POLYACRYLIC ACID 5100. CRYSTALS WERE SOAKED WITH 20.6 MM DON FOR 90 MIN |
