2V4O
Crystal structure of Salmonella typhimurium SurE at 2.75 angstrom resolution in monoclinic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.081, 95.299, 94.572 |
| Unit cell angles | 90.00, 98.93, 90.00 |
Refinement procedure
| Resolution | 93.250 - 2.710 |
| R-factor | 0.19271 |
| Rwork | 0.190 |
| R-free | 0.25428 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j9j |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.801 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 37552 | |
| <I/σ(I)> | 14.3 | 2.32 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES (PH 6.5) AND 12% PEG 20,000 |
